The Tetrahymena telomerase p75–p45–p19 subcomplex is a unique CST complex
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چکیده
منابع مشابه
Tetrahymena telomerase is active as a monomer.
Telomerase is an enzyme that utilizes an internal RNA molecule as a template for the extension of chromosomal DNA ends. The catalytic core of telomerase consists of the RNA subunit and a protein reverse transcriptase subunit, known as telomerase reverse transcriptase (TERT). It has previously been shown that both yeast and human telomerase can form dimers or multimers in which one RNA in the co...
متن کاملMultiple DNA-binding sites in Tetrahymena telomerase
Telomerase is a ribonucleoprotein enzyme that maintains chromosome ends through de novo addition of telomeric DNA. The ability of telomerase to interact with its DNA substrate at sites outside its catalytic centre ('anchor sites') is important for its unique ability to undergo repeat addition processivity. We have developed a direct and quantitative equilibrium primer-binding assay to measure D...
متن کاملTemplate boundary definition in Tetrahymena telomerase.
Telomerase elongates chromosome ends by addition of telomeric DNA repeats. The telomerase ribonucleoprotein can copy only a short template sequence within the telomerase RNA subunit. Here, we identify a region of telomerase RNA that is necessary for both correct 5' template boundary definition and high affinity telomerase reverse transcriptase (TERT) interaction. We also demonstrate that TERT m...
متن کاملTetrahymena telomerase ribonucleoprotein RNA-protein interactions.
Telomerase is an enzyme that is essential for the replication and maintenance of chromosomal termini. It is a ribonucleoprotein consisting of a catalytic subunit, one or more associated proteins, and an integral RNA subunit that serves as a template for the synthesisof telomeric repeats. We identified a Tetrahymena telomerase RNA-protein complex by an electrophoretic mobility shift assay, using...
متن کاملStructure and folding of the Tetrahymena telomerase RNA pseudoknot
Telomerase maintains telomere length at the ends of linear chromosomes using an integral telomerase RNA (TER) and telomerase reverse transcriptase (TERT). An essential part of TER is the template/pseudoknot domain (t/PK) which includes the template, for adding telomeric repeats, template boundary element (TBE), and pseudoknot, enclosed in a circle by stem 1. The Tetrahymena telomerase holoenzym...
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ژورنال
عنوان ژورنال: Nature Structural & Molecular Biology
سال: 2015
ISSN: 1545-9993,1545-9985
DOI: 10.1038/nsmb.3126